micro ct scanning  (Skyscan Corporation)

Journal: Bioactive Materials

Article Title: Biodegradable Mg 2+ -releasing piezoelectric scaffold for segmental bone defect repair

doi: 10.1016/j.bioactmat.2026.02.017

Figure Lengend Snippet: Preparation and characterization of Mg 2+ -releasing piezoelectric scaffolds. A) Schematic illustration showing the preparation process for PWH Gel. B) XRD patterns and C) FTIR spectra of WH NP and PWH NP. D) Representative SEM image and elemental mapping of PWH NP, demonstrating uniform distribution of the characteristic Ca, P, O and Mg element. E) 1 H NMR spectra of gelatin and GelMA. F) Representative SEM images and elemental mapping of PWH Gel, showing highly interconnected porous architecture and homogeneous element dispersion. G) Micro-CT reconstruction illustrating the three-dimensional interconnected porous structure of PWH Gel. H-I) Voltage and current outputs of the WH Gel and PWH Gel under pressure. J-K) COMSOL finite element analysis simulation showing the stress and electric field distribution under external compression. L) Photograph showing the piezoelectric response effect demonstrated by lighting a bulb. M) In vivo piezoelectric testing in a rat radial defect under cyclical compression at 10 N.

Article Snippet: The collected radius samples were scanned and imaged using a micro-CT scanner (SkyScan1276, Bruker, China) and a digital X-ray machine (Parameter 3D, Kubtec, USA).

Techniques: Dispersion, Micro-CT, In Vivo

Journal: Bioactive Materials

Article Title: Biodegradable Mg 2+ -releasing piezoelectric scaffold for segmental bone defect repair

doi: 10.1016/j.bioactmat.2026.02.017

Figure Lengend Snippet: In vivo assessments of large segmental bone defect regeneration using Mg 2+ -releasing piezoelectric scaffold. A-B) Schematic showing the surgical procedure for scaffold implantation in rat radial defects (Scale bar = 1 cm). C) Macroscopic images of the defect site at 6- and 12- weeks post-implantation. D) RUS scores for radial repair. E) 3D micro-CT images of the defects at 6- and 12- weeks post-implantation (Scale bar = 3 mm). F-G) Quantitative micro-CT analysis of BV/TV and trabecular number (Tb.N) in cryogel-treated regions at 6- and 12- weeks post-implantation. H) Representative H&E and Masson's trichrome staining images of defect tissues at 6- and 12-weeks post-implantation (Scale bar: 1 mm). I) Immunohistochemical staining for COL-I (Scale bar: 1 mm). J) Representative immunofluorescence staining of CD31 (Scale bar: 1 mm). Data are expressed as mean ± S.D. (n = 3 independent replicates). Statistical significance was determined as ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001; NS, not significant.

Article Snippet: The collected radius samples were scanned and imaged using a micro-CT scanner (SkyScan1276, Bruker, China) and a digital X-ray machine (Parameter 3D, Kubtec, USA).

Techniques: In Vivo, Micro-CT, Staining, Immunohistochemical staining, Immunofluorescence

Journal: International Dental Journal

Article Title: METTL1 Aggravates Periodontitis Progression by Inhibiting Osteoblast Differentiation via m7G modification of SEMA4D

doi: 10.1016/j.identj.2026.109498

Figure Lengend Snippet: Knockdown of METTL1 suppresses alveolar bone loss and inflammatory responses in a mouse model of periodontitis. (A-B) The transfection efficiency was detected by qPCR and Western blot. (C) Histological analysis of the periodontium using haematoxylin and eosin (H&E). Micro-CT (computed tomography) analysis of bone resorption state. (D-E) Quantification analysis of distance between cementum-enamel junction (CEJ) and BV/TV. (F-J) The levels of TNF-α, IL-1β, IL-6, IL-10, and TGF-β were evaluated by ELISA. (n = 6). All data are expressed as the means ± SD. ⁎⁎ P < .01.

Article Snippet: Fixed maxillae were mounted in paraffin wax (Leica Biosystems, Buffalo Grove, IL, USA) and scanned using a SkyScan 1272 High-Resolution Micro-CT System (Bruker MicroCT, Kontich, Belgium).

Techniques: Knockdown, Transfection, Western Blot, Micro-CT, Computed Tomography, Enzyme-linked Immunosorbent Assay

Journal: Bioactive Materials

Article Title: Sulfated polysaccharide prevents senescent adipocyte-driven osteonecrosis by stem cell fate reprogramming

doi: 10.1016/j.bioactmat.2025.11.039

Figure Lengend Snippet: Continuous intraosseous administration of SCS prevents glucocorticoid-induced bone degeneration. ( A ) Schematic illustration of the glucocorticoid (GC; MPS)-induced bone deterioration and intraosseous SCS treatment. ( B-D ) Representative H&E staining images of the femur at 6 weeks (B). Magnified views of the cortical bone and trabecular bone in the marrow cavity are shown on the right. Solid arrows indicate normal osteocytes, while hollow arrows indicate empty osteocyte lacunae. Quantification of empty lacunae ratios in cortical bone (C) and trabecular bone (D). n = 6 biological replicates. (Scale bars, 500 μm and 25 μm) ( E-H ) Representative immunofluorescence staining of OPN + mature osteoblasts, osteolectin + osteoprogenitors, and VE-cadherin + endothelial cells (ECs) in femur at 6 weeks (E), and corresponding quantifications (F–H). n = 6 biological replicates. (Scale bars, 100 μm and 20 μm) ( I and J ) Representative flow cytometry plots of capillary subtypes in the femur (I), with quantification of CD45 − Ter119 − CD31 hi Emcn hi ECs (J). n = 6 biological replicates. ( K and L ) Flow cytometry plots showing Sca-1 hi CD31 hi arteriolar ECs (K), and corresponding quantification (L). n = 6 biological replicates. ( M and N ) Representative micro-CT 3D images of the femur (M). Quantitative analysis of percent bone volume (BV/TV) (N). n = 6 biological replicates. (Scale bars, 1.5 mm, 600 μm and 545 μm) ( O and P ) ELISA analysis of VEGF (O) and PDGF-BB (P) levels in bone marrow supernatant and peripheral serum from PBS- and SCS-treated groups at week 6. n = 6 biological replicates. ( Q ) ELISA quantification of the osteogenic factor osteocalcin in peripheral serum at week 6. n = 6 biological replicates. Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns, not significant. Statistical significance was determined using one-way ANOVA with Tukey's post hoc test ( C, D, F, G, H, J, L, N, O, P and Q ).

Article Snippet: High-resolution micro-computed tomography (Micro-CT) scanning was performed using the Skyscan 1272 system (Skyscan).

Techniques: Staining, Immunofluorescence, Flow Cytometry, Micro-CT, Enzyme-linked Immunosorbent Assay

Journal: Bioactive Materials

Article Title: Sulfated polysaccharide prevents senescent adipocyte-driven osteonecrosis by stem cell fate reprogramming

doi: 10.1016/j.bioactmat.2025.11.039

Figure Lengend Snippet: SCS targets downstream senescent lineage commitment of bone marrow MSCs to mitigate GC-induced bone deterioration. ( A ) Schematic diagram illustrating the experimental design: CD45 − Ter119 − CD31 − LepR + MSCs isolated from mice co-treated with SCS and MPS for 7 days were subjected to in vitro lineage-competitive differentiation, followed by DEX-induced senescence in lineage-mixed cells. These cells were then adoptively transplanted into healthy bone marrow cavity to assess bone deterioration development. ( B ) Representative H&E-stained images of the femur 12 weeks after adoptive transfer. PBS-DEX group: LepR + MSCs from PBS and MPS co-treated mice subjected to in vitro lineage differentiation and DEX-induced senescence, followed by transplantation. SCS-DEX group: LepR + MSCs from SCS and MPS co-treated mice processed similarly. PBS group: solvent control without cell transplantation. Solid arrows indicate intact osteocytes; hollow arrows indicate empty lacunae. (Scale bars, 250 μm and 25 μm) ( C – E ) Quantitative analysis of marrow hypertrophic adipocyte diameter (C), proportion of empty osteocyte lacunae in trabecular bone (D), and adipocyte number (E) in the metaphysis 12 weeks post-transplantation. n = 19 biological replicates (C), n = 6 biological replicates (D), n = 8 biological replicates (E). ( F ) Quantification of empty lacunae in epiphysis at 12 weeks post-transplantation. n = 6 biological replicates. ( G – I ) Representative flow cytometry plots of capillary ECs subtypes in the femur at 12 weeks (G), with quantification of CD45 − Ter119 − CD31 hi Emcn hi ECs (H) and CD45 − Ter119 − CD31 lo Emcn lo ECs (I). n = 6 biological replicates. ( J and K ) Representative flow cytometry plots (J) and corresponding quantification (K) of CD45 − Ter119 − Sca-1 hi CD31 hi arteriolar ECs in the femur at 12 weeks post-transplantation. n = 6 biological replicates. ( L ) Representative micro-CT images of the femur at 12 weeks post-transplantation across different treatment groups. (Scale bars, 1.5 mm and 500 μm) ( M – P ) Quantitative analysis of bone parameters in the metaphysis: bone mineral density (BMD) (M), percent bone volume (BV/TV) (N), trabecular separation (Tb.Sp) (O), and trabecular number (Tb.N) (P). n = 6 biological replicates. ( Q ) Serum ELISA analysis of the osteogenic marker osteocalcin at 12 weeks post-transplantation. n = 6 biological replicates. ( R and S ) ELISA analysis of PDGF-BB (R) and VEGF (S) in both bone marrow supernatant and peripheral serum at 12 weeks post-transplantation. n = 6 biological replicates. Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001; ns, not significant. Statistical significance was determined using one-way ANOVA with Tukey's post hoc test ( C, D, E, F, H, I, K, M, N, O, P, Q, R and S ).

Article Snippet: High-resolution micro-computed tomography (Micro-CT) scanning was performed using the Skyscan 1272 system (Skyscan).

Techniques: Isolation, In Vitro, Staining, Adoptive Transfer Assay, Transplantation Assay, Solvent, Control, Flow Cytometry, Micro-CT, Enzyme-linked Immunosorbent Assay, Marker

Journal: Materials Today Bio

Article Title: Synergistic osteogenesis, angiogenesis, and immune reprogramming by a metal-phenolic functionalized electrospun fibrous membrane for alveolar bone regeneration

doi: 10.1016/j.mtbio.2026.103045

Figure Lengend Snippet: In vivo therapeutic effect of the EFMs in a rat alveolar bone defect model. A) 3D reconstruction and sectioned views of the rat maxillary alveolar bones. B, C) Quantitative results calculated from micro-CT data: CEJ-ABC distance, BV/TV. D, E) H&E staining and quantitative analysis. F, G) Masson's trichrome staining and quantitative analysis. H-J) The immunofluorescence images and quantitative results of OPN and VEGF. K) Schematic illustration depicting the animal model establishment and the experimental process. Data are presented as mean ± SD. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001; ns, not significant.

Article Snippet: The harvested maxillae were scanned using a micro-computed tomography (micro-CT) system (SkyScan1276, Bruker, USA) to evaluate alveolar bone regeneration.

Techniques: In Vivo, Micro-CT, Staining, Immunofluorescence, Animal Model